Journal: bioRxiv

Article Title: Diet-induced phospholipid remodeling dictates ferroptosis sensitivity and tumorigenesis in the pancreas

doi: 10.1101/2025.04.04.645324

Figure Lengend Snippet: (A) Disease burden (% disease/total pancreas area) for male KC mice fed control diet (CD) or designated HFDs for 12 weeks. Box plots designate 25 th , 50 th , and 75 th percentiles +/- min/max, n = 4-12 mice/diet. * p < 0.05, ** p < 0.01, Kruskal-Wallis test with Dunn’s post-hoc test. (B) Positive correlation between median disease burden of mice in (A) and dietary oleic acid concentration (µg/mg diet). Pearson correlation coefficient (R) and p -value are shown. (C) Stronger positive correlation between median disease burden of mice in (A) and mean total plasma oleic acid levels (nmol of FA/mL plasma) in non-tumor-bearing C57BL/6 male mice fed each diet for 12 weeks (n = 5 mice/diet). Pearson correlation coefficient (R) and p -value are shown. (D) Representative H&E and Ck19 (duct tumor marker) immunostaining of pancreata of male KC mice fed CD, LODs, or HODs for 12 weeks. Scale bars are 50 µm. (E) Disease burden (mean +/- s.e.m., n = 7-12 mice/diet) for male KC mice fed CD, LODs, or HODs for 12 weeks. * p < 0.05, ** p < 0.01, Kruskal-Wallis test with Dunn’s post-hoc test. (F) Proportional area (mean percentage +/- s.e.m., n = 6-9 mice/diet) of total disease by lesion type (ADM, PanIN, PDAC) of KC mice fed CD, LODs, or HODs. Proportions are not significantly different across diets, Kruskal-Wallis test with Dunn’s post-hoc test.

Article Snippet: The following primary antibodies were used: mouse anti-Ck19 (DSHB TROMA-III, 1:500), rabbit anti-Ki67 (Biocare Medical CRM325, 1:200), rat anti-p19 ARF (Santa Cruz Biotech sc-32748, 1:100), rat anti-Cd45 (Abcam ab25386, 1:100), rabbit anti-F4/80 (CST 70076, 1:400), rabbit anti-SMA (Invitrogen PA1-37024, 1:100), mouse anti-B220 (BD Pharmingen 550286, 1:40), rabbit anti-Cd3 (CST 78588, 1:200), rabbit anti-4-HNE (Abcam, ab46545, 1:200), and rabbit anti-Nqo1 (Sigma-Aldrich HPA007308, 1:100).

Techniques: Control, Concentration Assay, Marker, Immunostaining

Journal: JAMA Facial Plastic Surgery

Article Title: Use of Condensed Nanofat Combined With Fat Grafts to Treat Atrophic Scars

doi: 10.1001/jamafacial.2017.1329

Figure Lengend Snippet: A and B, Under Fontana-Masson staining, an increase in melanin is seen in the basal cell layer between the preoperative (A) and 6-month postoperative (B) specimens (original magnification ×400). C and D, Under cytokeratin (CK) 14 staining, almost no sebaceous or sweat glands were observed preoperatively (C), but they were clearly visualized 6 months postoperatively (D) (original magnification ×200). E and F, Under CK19 staining, almost no sebaceous or sweat glands were observed preoperatively (E), but they were clearly visualized 6 months postoperatively (F) (original magnification ×100).

Article Snippet: Following citrate incubation and blocking with 5% rabbit serum for 30 minutes, slides were incubated at 4°C overnight with the following antibodies against cytokeratin (CK) 14 and CK19 (both at 1:200 concentration; ProteinTech).

Techniques: Staining

Journal: STAR Protocols

Article Title: Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry

doi: 10.1016/j.xpro.2024.103018

Figure Lengend Snippet: Keratinocyte panel

Article Snippet: Rabbit polyclonal anti-mouse cytokeratin 6A (1:300) , Proteintech , Cat#10590-1-AP, RRID: AB_2134306.

Techniques: Concentration Assay, Staining

Journal: STAR Protocols

Article Title: Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry

doi: 10.1016/j.xpro.2024.103018

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse cytokeratin 6A (1:300) , Proteintech , Cat#10590-1-AP, RRID: AB_2134306.

Techniques: Recombinant, Staining, Cream, Software, Flow Cytometry

Journal: Animals : an Open Access Journal from MDPI

Article Title: Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

doi: 10.3390/ani10081400

Figure Lengend Snippet: Effects of calcium on the differentiation and proliferation of goat HMCs. Calcium dramatically changed the expressions of marker genes associated with keratinocyte differentiation on the mRNA ( a ) and protein levels ( b ). Counts of proliferating cells (green) accounting for the total cells (blue) visually increased ( c ), and the ratio significantly elevated with the rising calcium concentration ( d ). Moreover, calcium altered the cell cycle distribution of goat HMCs, although it did not meet a significance criterion ( e ). Note that only the protein level of KRT1 was confirmed in ( b ). **, p < 0.01 and *, p < 0.05; scale bar = 400 μm.

Article Snippet: Other routine reagents were previously preserved in our labs. For immunoblotting, we used the following antibodies: rabbit polyclonal antibodies for ALDH1A3 (1:1000), KRT1 (1:500), SOX21 (1:250), and β-actin (1:1000) (Proteintech, Chicago, IN, USA); rabbit polyclonal antibodies for FBP1 (1:2000), OVOL1 (1:1500), KRT23 (1:200), and HOXC13 (1:500) (Sangon Biotech, Shanghai, China); and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (H + L) (1:1000; Beyotime, Beijing, China).

Techniques: Marker, Concentration Assay